Recently, the team of Prof. Lu Hongbin of Department of Sports Medicine and Prof. Hu Jianzhong of Department of Spinal Surgery of Xiangya Hospital, Central South University published an original article titled “Engineering an enthesis-like graft for rotator cuff repair: An approach to fabricate highly biomimetic scaffold capable of zone-specifically releasing stem cell differentiation inducers” in Bioactive Materials(IF:14.593Q1). The study intends to optimize the current acellular protocol for engineering an enthesis-like graft for rotator cuff repair. Associate Researcher Chen Can from Department of Orthopedics, Xiangya Hospital, Central South University is the first author. Prof. Lu Hongbin and Prof. Hu Jianzhong are co-corresponding authors. Xiangya Hospital of Central South University is the sole affiliation of first author and corresponding author.
Clinically, rotator cuff tear (RCT) is common, leading to substantial pain and disability of the upper extremity, threatening the health of sports enthusiasts and professional athletes. In recent years, improved arthroscopic techniques have provided better results in the surgical management of RCT. Unfortunately, the regenerated enthesis after RCT repair often exhibits disorganized fibrovascular scars without triphasic yet continuous structure, and rarely recovers the tissue toughness, thus predisposing the repaired RC enthesis to re-injury.
Rapid and functional enthesis regeneration should stimulate the triphasic gradient enthesis formation of bone-fibrocartilage-tendon tissues, thus effectively preventing high re-tear rate after RCT repair. The most existing grafts bioengineered for RC repair healed with disorganized fibrovascular scar tissue instead of the formation of triphasic bone-fibrocartilage-tendon tissues.
Hence, based on a series of previous studies on book-shaped decellularized enthesis matrix(O-BDEM), the team fabricated an innovative vacuum aspiration protocol (2 granted national patents: ZL202010103858.6, ZL201911419374.6) and invented a novel biomimetic decellularized matrix scaffold with both morphological structure and mechanical properties. Using the stem cell differentiation-inducing active polypeptide with collagen-binding properties, the team managed to zone-specifically enhance the regeneration potential of bone, fibrocartilage, and tendon tissues of the novel decellularized matrix scaffold. The in-vitro constructed enthesis-like tissue’s efficacy in promoting the regeneration of the enthesis bone-fibrocartilage-tendon tissues triphasic structure was then verified through experiments on clinically similar large animal models. The study has resulted in a novel engineered graft for RC tear. (1 granted national patent: ZL201910796350.6)
The team of Prof. Lu Hongbin has been dedicated to studies on the enthesis repair mechanism and the enthesis regeneration strategy. Since 2015, the team has explored optimized protocols for the decellularization of compact tissues and the application of decellularized matrix materials in the repair of motor system tissue injury. Related research results were successively published in Bioactive Materials, Biomaterials, ACS Applied Materials & Interfaces, eLife, American Journal of Sports Medicine, Journal of Orthopaedic Research, and Journal of Orthpaedic Translation. 7 chinese patents were granted for the team’s research based on decellularized matrix materials. The series of studies also were supported by the National Key Research & Development Program of China, the Key Program/ the General Program/the Young Scientists Fund of NSFC, and the Major Science and Technology Project of Changsha. In the future, the team intends to leverage its decellularization technology for the development of a series of engineered tissue products with promising clinical transformation prospects for anterior cruciate ligament reconstruction, bone and ligament defect repair, meniscus repair, articular cartilage regeneration, and skin wound repair.
Link to Related Articles:
Xiangya Hospital Central South University
All Rights Reserved